Firms face several challenges related to sustainability in global logistics operations. In response to this new dynamic, logistics has evolved into what is now known as smart logistics or Logistics 4.0, which has been in constant change for the past 10 years. This evolution has occurred within the framework of Industry 4.0 and has been called to be the pillar that guarantees sustainability in firms through the development and implementation of innovative and disruptive technological solutions. A systematic analysis of the literature related to the role of logistics 4.0 in business sustainability in the agro-industrial sector was carried out. A bibliometric and content analysis was performed on the 56 publications identified from 2015 to 2021 in the Scopus, ScienceDirect and Springer databases. The bibliometric research method used was the joint analysis of keywords using VOSviewer, followed by a content analysis of the selected articles. The bibliometric analysis made it possible to identify that publication in this field is low and growing. Logistics 4.0 contributes considerably to the sustainability of firms in general, and even more to the agro-industrial sector through digital technologies and new business models, allowing the creation of competitive advantages in agro-industrial firms.